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吳郭魚耐鹽改良選育研究

  • 日期:107-02-20
  • 計畫編號:107農科-9.5.1-水-A1(2)
  • 年度:2018
  • 領域:漁業科技研發
  • 主持人:曾福生
  • 研究人員:杜金蓮、楊佳宜

    本研究以建立的紅色吳郭魚TsRn品系和海水吳郭魚TFS品系雜交之F1其組合有 TsRn♀×TFS♂和TFS♀×TsRn♂,其中TsRn♀×TFS♂組合在海水環境適應能力差,會 陸續全數死亡,反之TFS♀×TsRn♂在海水環境適應同TFS,比較F1分別回交TFS親本及 TsRn親本產下的BC1F1和F1自交的子代F2之耐鹽適應性 ,F2群幼苗到三月齡的存活率為 86%(3378/3939),有三種體色型態:紅底帶黑點、黑色群和紅底帶黑斑,其中只有 黑色群TFS♀/TsRn♂自交有苗產出F3幼苗到三月齡的存活率為90% (536/592)。正回 交BC1F1 (TFS/TsRn♀×TFS♂)和(TFS/TsRn♂×TFS♀)都有後代,所有魚苗體色全黑 ,(TFS/TsRn♀×TFS♂)組合三月齡的存活率為78% (559/713);(TFS/TsRn♂×TFS♀) 組合的存活率為81% (636/785);反回交 (TFS/TsRn♂×TsRn♀;TFS/TsRn♀×TsRn♂)無法在海水環境下配對,沒有後代產出 。 結果顯示海水適應性狀來自TFS,若TFS的血統低於1/2後代就有海水適應上的問 題。

研究報告摘要(英)


    In this study, the F 1line reciprocally crossed Taiwan red Nile tilapia(Oreochromis spp. )strain TsRn with the marine tilapia ( Oreochromis spirulus) strain, TsRn♀×TFS♂ and TFS♀×TsRn♂. One of them TsRn♀×TFS♂ got poor adaptability marine environment and die in succession. The other TFS♀×TsRn♂ had salt tolerance the same as TFS. Compare  F2 , F3 , and F1(TFS♀×TsRn♂) back crossed with parents TsRn♀, TsRn♂, TFS♀ and TFS♂ to produce BC1F1, respectively. The salt tolerance of F2 3-month-old progeny survival rate was 86% (3378/3939), and there were three color types: black dots, spots and black groups. Only the black group TFS♀×TsRn♂ produced F3 3-month-old progeny survival rate was 90% (536/592). The BC1F1(TFS/TsRn♀×TFS♂) and (TFS/TsRn♂×TFS♀) have progeny, all the fry are black, and 3-month-old survival rate of (TFS/TsRn♀×TFS♂) was 78% (559/713); (TFS/TsRn♂×TFS♀) was 81% (636/785); the other cross (TFS/TsRn♂×TsRn♀; TFS/TsRn♀×TsRn♂ ) can't reproduce in marine environment. The results show that the marine adaptation trait comes from TFS, and if it was lower than 1/2 TFS descent, the salt tolerance was a problem.