The aim of this project was to develop and improve the technology of grouper hybridization and to create new hybrid grouper species which have cold-resistant and fast-growing traits. We hybridized longtooth grouper ( Epinephelus brunneus), potato grouper (Epinephelus tukula) and kelp grouper (Epinephelus moara) egg with giant grouper (Epinephelus lanceolatus) milt, respectively and evaluated their economic trait (e.g. fast-growing) and stress tolerance (e.g. cold-resistant). Seven broodstock of giant grouper was sampled during February to March 2018. Two of them were collected the milt (viability over 80%). The eggs were collected from longtooth grouper, potato grouper and kelp grouper, however, the fertilization was 0%. The growth quadratic equation of body weight and total length of E. brunneus ×E. lanceolatus were Y=18.94- 6.49X+1.27X 2 (R 2= 0.963, P<0.001, n = 220) and Y=8.09+0.25X+0.07X 2 (R2 = 0.976, P<0.001, n = 220), respectively. The growth quadratic equation of body weight and total length of E. tukula x E. lanceolatus were Y=17.67- 9.7X+1.81X 2 (R 2= 0.972, P<0.001, n = 200) and Y=6.74+0.028X+0.12X 2 (R2 = 0.983, P<0.001, n = 200), respectively. In addition, compared with the cold-resistant ability for market size giant grouper, longtooth grouper and the hybrids (E. brunneus × E. lanceolatus) with plasma biochemical parameters analysis. The results showed that the cold-resistant ability of the hybrids was better than the giant grouper. The long-term goals of this project are to diversify the species of grouper aquaculture, and product the new economic grouper stably and promote new hybrid grouper to the aquaculture industry.