The aim of this study is to selective breeding YY supermale of nile tilapia ( Oreochromis niloticus ) and all-female nile tilapia group, used different methods tomass production of all-male offspring on a commercial scale. The test include two parts, which divides into five groups to sort out the female-reversed tilapia (XY△♀) and male-reversed tilapia (XX△♂). (1)The result of select female-reversed tilapia: the sex ratio of progeny in group 1 and group 2 are both 1:2.7, group 3 is 1:2.8. The chi square value of group 1 is 1.06; group 2 is 1.32 and group 3 is 0.91 with no significant difference of each groups ( p >0.05). The male ratio of group 1 is 73%; group 2 is 72.6% and group 3 is 73.3%, so the three groups female can be judged as a female-reversed tilapia (XY△♀). (2)The result of select male-reversed tilapia: The chi square value of group 1 and group 2 are 1189.03 and 517.01, which is significant difference of each groups ( p >0.001). The female ratio of group 1 and group 2 are 99.75% and 99.81%, so the two male can be judged as a male-reversed tilapia (XX△♂).