Pacific bluefin tuna is highly migration species with important commercial value. Their populations are overfishing due to the increasing numbers of removal from fishery in recent years. To analysis the trophic level, migrating and spawning behavior, we investigated the population structure of Pacific bluefin tuna by using the stable isotope analysis and electronic tagging in eastern Taiwan. Fishery data was collected from 2011 to 2017 and the range of standard length were from 200 to 220 cm, age was 15 in average. The stable isotope analysis results indicated that the trophic level of Pacific bluefin tuna was 5.1, belonging to top predator. Their δ 13 C value and δ 15 N value were different among months, and the differences could be related to the environmental factors such as primary production and water temperature of ocean. Additionally, we cooperated with the Hopkins Marine Station of Stanford University, to track the movements of Pacific bluefin tuna. We released 3 individuals with pop-up satellite archival tags, 5 with acoustic tags and 8 with archival tags. The study explored the trophic level and population structure of Pacific bluefin tuna in eastern Taiwan, and gained further understanding of the population structure and migrating behavior in western Pacific Ocean.