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基因改造水產動植物快速檢測技術開發與應用

  • 日期:106-03-04
  • 計畫編號:106農科-6.3.3-水-A1
  • 年度:2017
  • 領域:農業科技管理
  • 主持人:謝淑秋
  • 研究人員:葉信利、王俊堯、黃致中

收集及建立基因改造螢光斑馬魚(JYZR001)及基因改造螢光青鱂魚(TK-1)培育體系 ,並針對JYZR001及TK-1之轉殖基因設計專一性引子,分別成功以聚合酶連鎖反應 (polymerase chain reaction, PCR)技術夾取DsRed2(Discosoma sp. red fluorescent protein)和GFP(Green fluorescent protein)轉殖基因片段,建立基 因改造水產生物檢測樣品處理、萃取、純化與PCR定性檢測技術之標準作業流程。期 能提供完善的檢測技術供基因改造水產生物在未來上市後進行追蹤和管理,有效的 管控及減輕對環境生態造成之影響,並提升臺灣地區水產養殖生技產業之發展。  

研究報告摘要(英)


The objectives of this study are to collect and culture transgenic fluorescent zebrafish (JYZR001) and transgenic fluorescent medaka (TK-1), and to establish the standard operating procedures of sample processing, extraction, purification and polymerase chain reaction (PCR) technique for the qualitative detection. We designed the specific primers for JYZR001 and TK-1, and successfully cloned the gene fragments of DsRed2 ( Discosoma sp. Red fluorescent protein) and GFP (green fluorescent protein) by polymerase chain reaction (PCR). The integrity of detection techniques will provide for tracking and management of transgenic aquatic organisms (aquatic genetically modified organisms, aquatic GMOs) in the future to effectively control and mitigate the impact on the environment and enhance the development of the aquaculture industry in Taiwan.