(一)The study of hybridization and inbreeding of groupers：The purpose of this study is to investigate the availability of the hybridization of giant grouper ( Epinephelus lanceolatus ) and oblique-banded grouper ( Epinephelu smoara ). In early 2017, we have collected and conserve 15 giant groupers and 15 oblique-banded groupers for broodstock. From January 2017, we raised and controlled the culture temperature at 30 ℃ for giant groupers and later set the culture temperature at 26 ℃ for oblique-banded groupers in March 2017. All fish were fed with purple squids, skipjack tuna, and the Pacific saury to enrich their nutrition for reproduction. We treated fish with HCG (350〜450 IU/kg) and HHRH-A2 (25〜35 µg/kg) by injections in 2017. In April, we obtained 100 ml semen(n=1) from a male giant grouper. In May, We obtained 240 ml semen(n=1) from the same giant grouper and 4.4 Kg eggs(n=2) from two oblique-banded groupers. In July, a male giant grouper was obtained 100 ml semen (n=1)and a female produced 430 g eggs(n=1). However, the sperms and eggs could not fertilize. In August, we obtained 3.81 kg eggs(n=2) from female giant groupers. There were no semen or eggs obtained after hormones injection in October. (二)Cultivation and propagation of the warm water flounder, Paralichthys olivaceus ：The temperature stimulation successfully induces spawning and fertilization of the olive flounder ( Paralichthys olivaceus), with the fertilization rate of 81.2%. The fertilized eggs of the fish are buoyant and semi-transparent, with an average diameter of 0.94 ± 0.01mm (Mean ± SD). These eggs hatched at 32 h after fertilization(Temp. = 22 ± 1℃). The newly hatched larvae were about 2.12 ± 0.03 mm in total length. The larvae (3.47 ± 0.76 mm) were fed with rotifers ( Brachionus plicatilis ) from the 3rd day post hatching (DPH). The microalgae ( Nannochloropsis oculata ) were added to stabilize the water quality and nourish rotifers. The 6 DPH fry were about 4.58 ± 0.18 mm in total length. Artemia spp . were used as feeds for nutrition enrichment. The 20 DPH fry were about 6.56 ± 0.03 mm in total length. Then, the coronary fin on head grew rapidly. At this time, we continue to use the artemia for nutrition enrichment. The 24 DPH fry were about 8.38 ± 0.11 mm in total length, their tilted swimming with their eyes relocated. When the eyes were on the same side at 38 DPH, fry(13.88 ± 1.26 mm) began for metamorphosis and entered into the benthic stage. In nutrition experiments, poultry meal was used for the replacement of fish meal in feed to explore the growth effect of olive flounder. The results showed there were no significant difference of weight gains in the four test groups. It indicates that the poultry meal replacement could not enhance the growth in olive flounder. (三)Cultivation of the warm water fish,Girella punctata ：This study was divided into two parts. In the first trial, we adjusted the protein inclusion levels of the diets to feed G. leonina. Then, we collected the body length and body weight data to analyze the protein inclusion level with the best growth performance. The second trial was based on the results of the first trial, and we used chlorella powder to replace the fishmeal in the diets. We collected the body length and body weight data to analyze the optimal dosage of chlorella powder. In the first trial, the G. leonine juveniles with initial length 4.21±0.81 cm and initial weight 6.59±0.09 g were fed on five diets with different dietary protein inclusion levels of 30, 35, 40, 45 and 50% (CP30, CP35, CP40, CP45, CP50) for 6 weeks. It was found that the growth performance of CP40, CP45 and CP50 were significantly better than the other groups. The weight gains were 40.99 ± 1.69, 43.98 ± 2.22 and 43.09 ± 2.50% respectively, and the specific growth rates were 0.78 ± 0.03, 0.83 ± 0.03 and 0.81 ± 0.04% per day, respectively. Considering the cost of diets and growth performance, this study suggests that the best dietary protein inclusion level is 40%. In the second trial, the juveniles with initial length 4.66±0.92 cm and initial weight 6.64±0.22 g were fed on the experimental diets for 6 weeks. The diets were divided into five groups based on the chlorella powder replaced percentages of 0, 25, 50, 75, 100% (SW0, SW25, SW50, SW75, SW100). It was found that the growth performance of SW25 and SW50 were significantly better than the other groups. The weight gains were 47.65±1.53 and 44.09±1.84% and the specific growth rates were 0.89±0.02 and 0.83±0.03% per day . The broken-line regression shows the optimal dosage of chlorella powder is 26.5%. (四)The study of abalone hybridization：In this study, we investigated the effects of different diets on the growth of Haliotis diversicolor and H. discus hannai . The diets included Caulerpa lentillifera, Ulva lactuca , Sarcodia montagneana, Agardhiella subulata, Eucheuma serra, Botryocladia leptopoda, Gracilaria coronopifolia and salted Laminaria japonica . The dry meat weight of H. diversicolor fed with S. montagneana , G. coronopifolia and salted L. japonicawas higher, but the survival rate of H. diversicolor fed with salted L. japonica was relatively low. The mean wet weight of H. diversicolor fed with C. lentillifera ,S. montagneana ,E. serra and B. leptopoda was reduced. The dry meat weight of H. discus hannai fed with S. montagneana, G. coronopifolia and salted L. japonica was higher. The mean wet weight of H. discus hannai fed with the remaining diets was reduced. (五)The extraction and utilization of the ingredients of the macroalgae：In this study, we used Botryocladia leptopoda for developing cultivation, component analysis, extraction and other related technologies to promote the seaweed industry. Our results showed that the optimal weight gain rate 50.46±2.45% was obtained when Botryocladia leptopoda were cultivated in deep sea water containing 1μM ammonium salt at 25 ℃ and 10000 lux. We analyzed the basic nutrients composition of Botryocladia leptopoda which contained 94.4 grams water, 3.1 grams ash, 10 calories, 0.5 grams proteins, 2.0 grams carbohydrates(0.9 grams of dietary fibers) in each 100 grams algae. The algae did not contain sugars, fats, trans and saturated fats. In addition,Botryocladia leptopoda also contains glutamic acid, glycine, cysteine, sodium, potassium, magnesium, calcium, phosphorus, boron, iron, copper, nickel, zinc, manganese and cobalt. In the extraction experiment, we divided the test samples into untreated group, ice extraction group and hot extraction group. We found that hot extraction group is better than the other two groups in the aspects of total sugar, total phenol, reducing power, ability to scavenge superoxide anion, capacity to capture DPPH free radicals and ability to chelate ferrous ion. It suggests that Botryocladia leptopoda has good antioxidant capacity and potential for developing natural and safe anti-oxidant or anti-aging material via a simple extraction method.