農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本計畫針對本所澎湖中心所繁殖之德斑氏活額蝦為標的，透過次世代定敘及兩種不 同的de novo序列組裝進行簡單重複序列標記(SSR)的預測分析，並利用毛細管電泳 及螢光標記引子進行族群遺傳變異分析。以CLC軟體組裝的contig預測的SSR總數為 39,169，在SSR區域兩端具有100bp可設計primer的數量為19,536，可被raw reads覆 蓋率達100%的數量有17,366。以ABySS組裝的contig預測的SSR總數為133，在SSR區 域兩端具有100bp可設計primer的數量為45，可被raw reads覆蓋率達100%的數量有 33。。總共使用10組引子對進行於螢光引子毛細電泳分析技術(Capillary electrophoresis with fluorescent-labeled technique)進行遺傳歧異度分析。 PIC(Polymorphism information content)值界於0.38 至0.412。遺傳分化指數 (Fst)為 0.0184，小於0.05，顯示兩族群間無顯著遺傳分化。利用STRUCTURE軟體分 析出對偶基因的分布情形，結果顯示兩個族群無明顯的遺傳結構差異存在，但在庫 外組群的結構種類有較多的趨勢。

In this study, we hope to predict the SSR from two different de novo assembling methods and analysis the population genetic form wild and aquacultured stock. The total number of SSRs from CLC assembled contigs was 39,169, the number of SSR with adjacent 100 bp region was 19,536, which was 17,366 with 100% coverage of raw reads. The total number of SSRs from ABySS assembled contigs was 133, the number of SSR with adjacent 100 bp region was 45, which was 17,366 with 100% coverage of raw reads. A total of 10 primer pairs were subjected to genetic divergence analysis using a Capillary electrophoresis with fluorescent-labeled technique. PIC (Polymorphism information content) values range from 0.38 to 0.412. The genetic differentiation index (Fst) was 0.0184, less than 0.05, indicating no significant genetic differentiation between the two populations. The STRUCTURE software was used to analyze the distribution of the dual genes. The results showed that there was no obvious genetic structure difference between the two populations.