1.Cultivation of the grouper broodstock and seed production：This study is aimed at the investigation of production of the giant grouper ( Epinephelus lanceolatus) and oblique-banded grouper (Epinephelus moara) hybrids. In 2016, we have collected 20 giant groupers and 40 obliquebanded groupers. The giant groupers were raised in the temperature controlled tanks and fed with purple squids, skip jack tuna, and the Pacific saury. We treated the female oblique-banded groupers with HCG (350〜450IU/kg)and HHRH-A2 (25〜35 µg/kg) by injections in March 2016. We obtained 7.435 Kg eggs, but only 10 g reached to the final maturation stage. In June 2016, we used the giant grouper sperms to fertilize with oblique-banded grouper eggs. The fertilization rate was about 92% and the embryos were able to develop to hatch out. In November 2016, the obliquebanded groupers became thin and their bellies were slightly hard. On the other hand,the giant groupers released few milt when pressed it softly. 2.The technical development of propagation andcultivation of the abalone ( Haliotis discus hannai)：In this study we collected more than 1,000 mature abalones (Haliotis discus hannai). After selection and inbreeding for at least six months, we found those abalones that show no significant differences in shell length, shell width, body weight and growth rate. We also found that the reproductive glands in male and female abalone were well developed and ready for experiment. According to our experimental designs, we collected and compared with fertilization rate, hatching rate, and abnormal rate. The growth factors, including shell length, shell width, body weight and survival rate were also measured. We found that there were no significant differences in fertilization rate(>82%), hatching rate(>76%), and abnormal larvae rate(<12%) after artificial reproduction. The survival rate was more than 90% during 6 months. These data were measured from more than 3, 000 individuals at juvenile and young stages in abalone development. In conclusion,the culture of small abalone by using artificial facility was established in this experiment, it can solve the problem of inbreeding with bad environmental situation. We also collected the growth data base of the larvae of the small abalones cultivation. 3.Cultivation of the broodstock and fry of the coldwater flounder ( Paralichthys olivaceus)：This study was conducted to investigate the effects of different environments on the growth of olive flounder ( Paralichthys olivaceus), including different illumination gradients and substrate environments. The illumination gradients were divided into four groups. The olive flounders were cultured in the condition of 1500-2000 Lux without shade net , and in the shading conditions with 800-1000 , 200-300 and 20-30 Lux, respectively. The results showed that the optimum weight gain rate was 309.09 ± 1.58% in 800-1000 Lux group, followed by 200-300, 1500- 2000, 20-30 Lux groups, which there were no significant difference in the last three groups. In the substrate experiment,the olive flounders were cultured in a FRP tank without bottom sand and a FRP tank with bottom sand. The results showed that the weight gain rate was 30.75 ±1.05%, which was significantly higher than the bottom sand group (14.90 ±1.95%). We conclude that the olive flounder can be cultivated and stably growup with the appropriate lighting and in the FRP tank without substrate. 4.Cultivation and utilization of the large algae：In this study, we used Caulerpa lentillifera for developing cultivation, extraction and other related technology to promote the seaweed industry. Our results showed that the optimal weight gain rate 10.18% was obtained when Caulerpa lentillifera were cultivated at 20 ℃,5000 lux and deep sea water with active liquid fertilizers. We analyzed the basic nutrients compositionof Caulerpa lentillifera which contained 94.6 grams water, 3.2 grams ash, 7.6 calories,0.4 grams proteins, 1.8 grams total carbohydrates(0.6 grams of dietary fibers),and 965 milligrams of sodium in each 100 grams algae. The algae did not contain sugars, fats, trans-formed and saturated fats.
In the extraction experiment, 20g fresh algae were extracted by four treatments (grinding, liquid nitrogen,liquid nitrogen + heating, liquid nitrogen + shaking), the sugar content obtained was 9.07, 10.90, 15.99 and 13.43 mg, respectively. We adjusted the above four crude extracts concentration as 0.88mg / ml and compared the reducing power and the clearance superoxide anion. Our results showed the best reducing power was obtained by the extraction of liquid nitrogen and the best clearance of superoxide anion was obtained by the direct grinding(30.61%). In the future we will try various algae cultivations, develop more products and extract other useful substances in order to increase the value and effectiveness of seaweed applications for the economy. 5.Collection and cultivation of the food organisms：In this study, we investigated the effects of different illumination time, pump air volume and inoculation ratio on the culture of Tetraselmis chui.According to the results, it is suggested to use 24 hours light per day, 5 liters per minute pump air volume and 3L algal source with 7L seawater forsmallcapacity (10L) culture. For large-capacity (80L) culture, it is suggested to use 20 liters per minute pump air volume and 15L algal source with 65L seawater. The effects of different diets and feeding frequencies on the culture of Brachionus ibericus and Apocyclops royi were also studied. B. ibericus fed with Nannochloropsis oceanica only at the beginning of the test could reach the density of 570 individuals/ml on the 5th day. A. royi fed with Tisochrysis lutea every 3 days could reach the density of 5 individuals/ml on the 9th day.