農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

本研究為收集鬼頭刀腦下垂體並初步試驗使用鬼頭刀腦下垂體進行日本鰻(Anguilla japonica)人工催熟下觀測其生殖腺發育程度變化。本年度收集鬼頭刀，其雌魚生殖 腺指數介於0.49-5.8之間，平均為3.39±1.17；雄魚生殖腺指數介於0.24-1.60之間 ，平均為0.54±0.21。鬼頭刀生殖腺經組織切片結果，生殖腺指數較高的雌魚，其生 殖腺中以卵黃堆積後期卵細胞居多，而生殖腺指數較低的雌魚，生殖腺中則以初級 卵母細胞為主。因此鰻魚人工催熟試驗為選擇生殖腺成熟的腦下垂體樣本，來作為 人工催熟材料。使用鬼頭刀腦下垂體進行鰻魚人工催熟試驗結果，鰻魚在注射第6- 7針後其胸鰭開始變黑，且腹部柔軟。在注射13針次後，鰻魚生殖腺成熟指數平均為 2.01±0.21。未來將持續探討鬼頭刀腦下垂體對鰻魚催熟效果。

In this study, the pituitary glands were collected from dolphin fish, Coryphaena hippurus and subjected to the artificial induction of Japanese eels (Anguilla japonica) maturation. The gonadosomatic index(GSI) of female Coryphaena hippurus ranged from 0.49-5.8 with an average of 3.39 ± 1.17, and GSI of male Coryphaena hippurus ranged from 0.24 to 1.60 with an average of 0.54 ± 0.21. The histology of the Coryphaena hippurus gonads showed that more oocytes were developed into the vitellogenic stage in those fishes which had higher GSI. The gonads stayed mainly in the primary oocyte stage in those fishes which had the lower GSI. We used the pituitary glands of the fishes which had higher GSI and then performed the artificial maturation of Japanese eels. The results showed that the pectoral fins of the eels began to darken and bellies became soft after 6-7 injections of the female dolphin fish pituitaries. The average GSI of eel was 2.01 ± 0.21 after 13injections of the female dolphinfish pituitaries. We will then evaluate the effect of the artificial maturation on the Japanese eels with Coryphaena hippurus pituitary .