The purpose of this project is aimed at the establisment of the gynogenesis technology for the Cyprinidae and is also expected to provide the experimental information for other economic fish species and femilization of fish in the future. Comet goldfish and goldfish which belong to Carassius auratus have variant figures and body colors due to long term breeding and selection. We will culture the 10 mature males and females of both fish in two tanks. In the meantime, we will observe their spawning behaviour and collect the fertilized eggs for developmental observation. Comet goldfish and goldfish will be seperated in two tanks before collecting the eggs and milt. The collected milt will be mixed with freshly prepared Hank's solution and then irradiated for 2 mins by using UV crosslinker. The inactivated sperms will subject to the collected eggs for artificial fertilization. We used 6 gene locus for haplotype analysis in which the results indicated that the ratio of gynogenesis among the progenies was about 1~2%.