農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

    建立臺灣主要養殖石斑魚(鞍帶石斑、點帶石斑及棕點石斑等)之精液簡易冷凍 保存程序與方法，尋求最適當且符合業者需求之保存方式。建立石斑精子銀行。運 用調控產卵技術及其冷凍精液，開發石斑雜交之繁養殖技術及精進其育苗技術，推 廣產業應用，帶動石斑魚產業精緻化發展。    本研究共冷凍保存石斑精液試驗12批次(鞍帶石斑4批次、點帶石斑1批次、雲紋 石斑2批次及棕點石斑5批次)；試驗結果顯示使用Cryo 1℃ Freezing Containers填 充異丙醇放置-80℃冰箱中4小時取代以液態氮蒸氣階段式進行降溫方法較為簡易 ，但如需長期保存仍需置放於-196℃液態氮中。    此外，以雲紋石斑、鞍帶石斑、棕點石斑及點帶石斑，進行生殖調控及石斑冷凍 精液雜交育種試驗，共計10次，受精率自11.52~90.32%，孵化率12.86~87.35%，畸 形率86~70.42%；以鞍帶石斑冷凍及新鮮精液與點帶石斑進行雜交育苗，結果顯示冷 凍精液與新鮮精液其受精率及孵化率差異不明顯，畸形率以冷凍精液組較低；以棕 點石斑冷凍及新鮮精液進行育苗試驗，結果顯示冷凍精液與新鮮精液對照組其受精 率、孵化率及畸形率，均以冷凍精液組較佳。最後，本計畫以凍結保存之棕點石斑 冷凍精液進行人工繁殖，134天後育成232尾仔魚，平均體長15cm、體重54.40g。

Establishing a simple method and procedure of cryopreservation for the main aquaculture grouper species (Epinephelus lanceolatus、E. coioides and E. fuscoguttatus , etc.) in Taiwan.  Finding the most appropriate preserving method that meets the needs of the industry. Establishing a grouper sperm bank. We will develop and improve the breeding technology of grouper hybridization by regulating spawning behavior and semen cryopreservation. It can increase the industrial applicability and improve the advanced development of the grouper industry.

In this study we performed 12 batches of grouper cryopreservation, include Epinephelus lanceolatus 4 batches, E. coioides 1 batch, E. moara 2 batches and E. fuscoguttatus 5 batches. The results confirmed it’s simple and efficient that use Cryo 1℃ Freezing Containers filled with isopropanol and place in -80℃ refrigerator than use liquid nitrogen and gradually cooling, but if we want to store in long terms, it must be stored with liquid nitrogen in -196℃.

In addition, we performed 10 trials of grouper artificial propagation, insemination rate is 11.52~90.32%, hatching rate is 12.86~87.35% and deformity rate is 86~70.42%. The experiment result shows that if use the seminal fluid of Epinephelus lanceolatus to carried out artificial propagation with Epinephelus coioides, there is no significant difference in insemination rate and hatching rate between frozen and fresh seminal fluid, but in deformity rate, using frozen seminal fluid is better than using fresh seminal fluid. Similar to previous results, use the seminal fluid of Epinephelus fuscoguttatus to carried out artificial propagation, using the frozen seminal fluid is better than using fresh seminal fluid in insemination rate, hatching rate and deformity rate.

This project using the frozen seminal fluid of Epinephelus fuscoguttatus to carried out artificial propagation and produce 232 Larvae, cultivate after 134 days, the average body length is 15cm, the average weight is 54.40g.