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極端溫度環境對微藻培養之影響

  • 日期:110-08-02
  • 計畫編號:109農科-9.3.3-水-A1(7)
  • 年度:2020
  • 領域:漁業科技研發
  • 主持人:許自研
  • 研究人員:黃欣梅

微藻種類繁多,可廣泛應用在水產養殖、食品製造、生質能源或是水處理及固碳等 方面。近年來,因氣候變遷日益加劇導致全球氣溫呈現極端化發展,嚴重影響藻類 生長進而使應用成效下降。為面對極端氣候之挑戰,本計畫嘗試開發可供各界應用 之藻原,從自然水體篩選出具極端冷、熱抗性潛力之微藻,探討其對溫度逆境之適 應能力,以擴增可利用微藻之應用特性,為後續研究及應用奠定基礎。本試驗自野 外採回藻水,以恆溫生長箱進行控溫培養,再進行分離純化,共篩選出5株微藻,分 別為圓篩藻之U1及S35、直鏈藻ME、菱形藻NI以及矽藻R1,目前試驗結果顯示U1極端 溫度適應範圍為5-36℃;S35極端溫度適應範圍為5-39℃;ME極端溫度適應範圍為5- 36℃;NI極端溫度適應範圍為5-30℃;R1極端溫度適應範圍為5-39℃,該等藻株在 低溫情況下雖然生長緩慢甚至停滯但並未死亡,一旦恢復室溫便能正常生長;在高 溫培養下,除NI以外之藻株初期皆有快速成長的趨勢,但因矽藻生活史較短,容易 快速增殖亦會急遽衰退,故高溫培養中期濃度開始下降減少。在藻體成分組成方面 :水分含量以R1最高(19.12±0.31%);灰份含量亦係R1最高(23.83±0.75%);粗蛋白 質含量以ME為最高(39.70±0.39%);脂質含量則以S35為最高(23.62±0.08%),透過試 驗結果可知,雖然U1及S35皆為圓篩藻,但其生長特性、溫度適應能力以及營養組成 卻有相當大的差異。故此,藉由人為調控培養條件並進行藻株定向選殖,可獲得具 理想特性或應用潛力之藻株。

研究報告摘要(英)


There are many kinds of microalgae, which can be widely used in aquaculture, food manufacturing, biomass energy or water treatment, and carbon sequestration. In recent years, due to the increasingly intensified climate change, the global temperature has shown extreme development, which has severely affected the growth of algae and reduced the effectiveness of the application. To face the challenge of extreme weather, this project attempts to develop algae that can be used by all walks of life, select microalgae with extreme cold and heat resistance potential from natural water bodies, and explore their ability to adapt to temperature adversity to expand their potential Use the application characteristics of microalgae to lay the foundation for subsequent research and application. In this experiment, the microalgae were collected from the field, cultured in a constant temperature growth box, and then separated and purified. A total of 5 strains of microalgae were screened out, namely, Coscinodiscus U1 and S35, Melosidopsis ME, Nitzschia NI, and Diatom R1, the current test results show that U1 extreme temperature adaptation range is 5-36℃; S35 extreme temperature adaptation range is 5-39℃; ME extreme temperature adaptation range is 5-36℃; NI extreme temperature adaptation range is 5-30 ℃; the extreme temperature adaptation range of R1 is 5-39℃. Although these algae strains grow slowly or even stagnate under low-temperature conditions, they do not die. Once the room temperature is restored, they can grow normally; under high-temperature cultivation, algae other than NI In the early stage of the plant, there is a trend of rapid growth, but due to the short life history of diatoms, it is prone to rapid proliferation and rapid decline, so the concentration of the high-temperature culture begins to decrease in the middle of the culture. In terms of algae composition: R1 has the highest moisture content (19.12±0.31%); ash content is also the highest R1 (23.83±0.75%); crude protein content is the highest with ME (39.70±0.39%); lipid content is the highest S35 is the highest (23.62±0.08%). The test results show that although both U1 and S35 are Coscinodiscus, their growth characteristics, temperature adaptability, and nutritional composition are quite different. Therefore, by artificially regulating the culture conditions and directional selection of them, algae strains with ideal characteristics or application potential can be obtained.