Lonicera japonica is rich in polyphenol compounds such as chlorogenic acid and has broad antibacterial activity. This project, therefore, evaluated the antibacterial activity of L. japonica extract against Streptococcus iniae in vitro and on disease resistances against streptococcosis in Lates calcarifer and Eleutheronema tetradactylum. The results showed that L. japonica extract has antibacterial activity against S. iniae and if the La. calcarifer was fed with L. japonica extract at doses of 0, 1, 2, 3 and 4% in feed for 14 days or E. tetradactylum was fed with L. japonica extract at doses of 0, 1, 2, 3 and 4% in feed for 28 days then challenged with S. iniae, the disease resistances of all added groups were significantly better than the control group (p<0.01) with the best protective abilities increased by more than 50%.