The oyster aquaculture is important fishery economical activities in the western coastal area of Taiwan. The major cultivated oyster species is the Portuguese Oyster (Crassostrea angulata ).How to collect high quality oyster spat is the most critical process of whole oyster culture industry. In this study, we will develop an oyster larvae transport model,and use field data to verify model simulation results, to forecast the optimal oyster spat collecting time. In addition, we will also investigate the oyster eyed larvae distribution layer. Moreover, to increase the success rate of oyster spat harvest, and increase the earnings of fisherman. Second, based on the monitoring and analysis of water environment data in Yunlin, Chiayi and Tainan oyster farming areas, the possible reasons for affecting the growth and opening of oysters were found out, and countermeasures or methods for improvement were developed. To improve the breeding efficiency and have higher economic benefits, the existing breeding mode is improved and combined with the cultchless oyster breeding method. In addition, uncultivated oysters (diploid, 2N) were collected, and literatures related to various oyster polyploids were collected. Referring to the test procedure of Pacific oysters (Crassostrea gigas), tests were carried out on Portuguese oysters (Crassostrea angulata ) from Taiwan.