This project aims to establish and optimize the breeding population of salt-tolerant tilapia, creating selection criteria for breeding material, and apply the whole genome of salt-tolerant tilapia to locate genetic markers for economic traits such as salt tolerance and growth. In addition, we will develop a second species of salt-tolerant tilapia for subsequent selection and breeding. Next, genomic breeding and gene stacking techniques are used to improve and enhance the stress tolerance of existing cultured species.