The Kuhliidae family (also known as flagtail fish) in Taiwan is consisted of one genus and three species, and Kuhlia rupestris is the largest among them. K. rupestris can be used as ornamental, food and game fish. K. rupestris sold in Taiwan’s aquarium and fish market are caught from the wild, so the supply chain is relatively unstable. This project intends to establish K. rupestris breeding and cultivating technologies. In addition to increasing the selectivity of fishermen to breed species, K. rupestris is also a native fish species that can withstand high temperature in response to climate change. In the first two years of this project, chip identification technology and juvenile feeding strategy have been established, and the effect of feeding raw bait on gonad development has been studied. This year, it is expected to establish the breeding technology to set up the dosage and timing of hormone injection, hoping to obtain fertilized eggs for fry cultivation.