Eleutheronema tetradactylum is currently an important fish species for marine fish farming in southern Taiwan. The main production areas are Jiadong and Fangliao in Pingtung County. In recent years, climate change and environmental changes in Taiwan have intensified, resulting in changes in the breeding environment. The fish need to spend more energy to adapt to the environment, bring about frailty. Especially after drastic changes in weather, the fish during the culturing period become getting bacterial epidemic and parasitic infection easier. During the breeding period, farmers often increase the stocking density and feeding frequency in order to increase the output value and shorten the breeding time. This kind of inappropriate breeding operation promotes the disease outbreak of fish.In order to improve the tolerance of fish to stressful adversity, this project intends to evaluate the effect of feed additives such as choline, tryptophan, carotenoids, vitamins C and E in enhancing the tolerance of fish to stressful adversity. These feed additives can enhance the fish body healthy and alleviate the stress on the breeding environment. By adding feed additives to feed to make functional feed,supplemented by breeding feeding technology, develop a breeding strategy tailored to local conditions, thereby reducing the use of drugs in breeding, and promoting healthy production of fish.