The east asian fourfinger threadfin, Eleutheronema tetradactylum, is the most important mariculture species in recent years in Taiwan. The demand of larvae is increasing greatly. The success rate of larval fish production in the industry is only about 20% and the quality of larvae is unstable. Leading to disease outbreaks in the subsequent cultivation period and the survival rate is decreasing. It also causes the improper drug use by fish farmers. In this study, we want to use high nutritional value microalgae (Isochrysis galbana, and Nannochloropsis oculata, etc.) and commercially available probiotics (Bacillus sp., etc.) to enrich live food of larval breeding for east asian fourfinger threadfin. It can improve the nutrition and also reduce the carry of pathogen for live food. Therefore, the success rate of larval production and the quality of seedlings are improved, so as to achieve the sustainable development of culture industry for the east asian fourfinger threadfin, Eleutheronema tetradactylum.