The quality of five finger thteadfin larvae is usually uneven and unstable. Stress during larviculture and transportation usually result in losses. High-quality seedlings are considered to be an important limiting factor for the development of aquaculture. Therefore, a "simple and reliable method" is needed to determine the quality of seedlings in farms or hatcheries, which will help improve aquaculture performance. This plan intends to use temperature, salinity and chemical reagents to make tolerance tests to evaluate the quality of larvae. In addition to evaluate the reproducibility of larvae rearing and nursery in this year, another major work item is to evaluate the quality of post larvae. The analysis items include the incidence of deformities, the number of parasites, PCR analysis of bacterial pathogens and anti-stress analysis of environmental factors.We wish these data can be used to establish an evaluation basis and establish a standard method as a reference for the quality evaluation of domestic healthy seedlings.