Seed production of cultured aquatic organisms is one of the most important issues forsustainable aquaculture industry in Taiwan. The culture collection center of live food isbuilt up to provide stock cultures and relative technology for farmers and researchers.To maintain the stock cultures (67 microalgae strains and 9 rotifers strain) subculturewere made every 2-12weeks. For continuously providing the 50ml-500l culture, massculture of microalgae and rotifer were practiced all year round. In addition tomaintaining the stock cultures (microalgae and rotifers) already hold, the collection,isolation and culture of other suitable live food will be conducted. Experiments forimproving culture technique and consultant of seed production technology will becontinued as well.