For improve the techniques of marine fish's culture and produce mass seed, we have tostudy the development of embryos, larval rearing, established the broodstock of parentfish, and selects the suitable species for Taiwan aquaculture. Hybridization is generallyuseful practice for species develop and keep good quality genetic strain in a role ofaquaculture. Grouper are important marine culture fishes in Taiwan. While not somuch research about those species hybrids study in now. Therefore, the research onphysiology and ecology about the hybrid seed culture are important. We have tried thepossibility of cross hybridization and the hatching of hybrid in black porgy (A. schlegeli)and gold lined sea bream (S. sarba). Then, we are going on the above study in larvaeunder different environmental factor treatment in future research. Furthermore, we willtry the practical technique to keep broodstock strain of grouper. We will get thedifference of morphology characters and growth data in juvenile /fry of grouper and ithybrids expect these basic studies will be very valuable for the mass seed production.