農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

水產種苗產業是臺灣養殖業永續發展中極重要之一項，而生物性餌料是魚、蝦、貝苗初期發育階段中不可欠缺的食物。為提供國內研究人員及水產養殖業者人工繁殖魚介幼生所需的餌料生物種原，建立水產餌料生物種原庫。種原庫中名為Chlorella 之10株種原，其EPA含量不等，顯然種名鑑定上不正確。由於綠球藻及擬球藻均屬5微米以下、圓形、且細胞表面無特徵之綠色藻類，從形態、色澤、鹽度耐性上難以區別。因此本研究將利用細胞核18S rDNA 基因序列來加以鑑定，以建立微藻分生鑑種技術，並取得海水綠球藻遺傳基因資訊。

Seed production of cultured aquatic organisms is one of the most important issues forsustainable aquaculture industry in Taiwan. The culture collection center of live food isbuilt up to provide stock cultures and relative technology for farmers and researchers.Ten culture collections of Chlorella had different EPA content in lipid, showed there arenot the same species. It is difficult to identify due to small size (< 5 μm), ovoid shape,without features on cell cover. To determine the relationships among Chlorella spp.strains, the PCR technique to analyze the nuclear-encoded SSU 18S rDNA sequencewill be used. In addition, this study is aimed to establish the technique for phylogeny ofmicroalgae culture collection.