1.For improve the techniques of marine fish's culture and produce mass seed, we haveto study the development of embryos, larval rearing, established the broodstock ofparent fish, and selects the suitable species for Taiwan aquaculture. Hybridization isgenerally useful practice for species develop and keep good quality genetic strain in arole of aquaculture. Grouper are important marine culture fishes in Taiwan. While notso much research about those species hybrids study in now. Therefore, the research onphysiology and ecology about the hybrid seed culture are important. We have tried thepossibility of cross hybridization and the hatching of hybrid in black porgy (A. schlegeli)and gold lined sea bream (S. sarba). Then, we are going on the above study in larvaeunder different environmental factor treatment in future research. Furthermore, we willtry the practical technique to keep broodstock strain of grouper. We will get thedifference of morphology characters and growth data in juvenile /fry of grouper and ithybrids expect these basic studies will be very valuable for the mass seed production.2.Although induced sex reversal and spawning in some species of grouper (giantgrouper, coral trout and mouse grouper⋯.) have achieved, but survival of the larvae inthe hatchery until early juvenile remains considerably low and unstable. It constrainsseriously the development of groupers aquaculture and seed export. The photosyntheticbacteria allow propagation to occur in anaerobic conditions and through respiration inaerobic condition. The photosynthetic bacteria (PSB), a single cell protein have strongability to break down ammonia and high nutrient value, contain many vital aminoacids, vitamins and carotenoids. In past, it was used in organic wastewater treatment toimproved environmental quality and clarification of sea water in fish cultivation, but lessapplication as a feed to fish larvae and zooplankton. Therefore, we will investigate thePSB to apply in grouper seed production and get some information about application ofPSB from this study. There knowledge should be helpful for improve seed productionindustry. In order to study the application of photosynthetic bacteria in groupers seedproduction, we will design a series of researches and divide to three parts. First, we useda series of methods for isolation and identification and also investigate the optimalgrowing condition of the photosynthetic bacteria, under different environmentalcondition. The second part of this study was to investigate the application ofphotosynthetic bacteria on growth of the rotifer and its dietary value for groupers.Third, was to experiment the effect of water quality on addition to groupers larvaerearing pond.3.In ontogeny of fish, metamorphosis is a phase between larval and juvenile periods.Elevated mortality rates during or shortly after metamorphosis have reported for manyfishes, and grouper are ones of them. The high mortality could be caused bydeteriorating water quality, viral infection, and cannibalism. Additionally, sincemetamorphosis includes major changes in morphology, either internal or external,unsuccessful transitions of those from larval to juvenile periods could makemetamorphosis quite hazardous time for the developing fish. However, so far, rarestudies concerning metamorphosis of grouper have been reported. Herein, we willinvestigate development of digestive tract and thyroid of grouper with histologicalmethods. Successful transitions of the two organ systems during metamorphosis are vitalfor grouper fry. The basic study should be helpful for larviculture of grouper.4.Seed production of cultured aquatic organisms is one of the most important issues forsustainable aquaculture industry in Taiwan. The culture collection center of live food isbuilt up to provide stock cultures and relative technology for farmers and researchers.To maintain the stock cultures (67 microalgae strains and 9 rotifers strain) subculturewere made every 2-12weeks. For continuously providing the 50ml-500l culture, massculture of microalgae and rotifer were practiced all year round. In addition tomaintaining the stock cultures (microalgae and rotifers) already hold, the collection,isolation and culture of other suitable live food will be conducted. Experiments forimproving culture technique and consultant of seed production technology will becontinued as well.5.The objectives of this study are: Culture and collection of copepod: Common speciesfound in fish ponds will be investigated. proper species will be selected and the culturemethod of using powder of artificial pellet or artificial fertilizer to replace microalgae ascopepod diet will be explored. To inhibit carried pathogens and virus of copepods byusing probiotics will be conducted.The expected results and their value: The economical viable culture method of copepodwill be established to decrease man-power needed during culture and to improve thequality of copepod to facilitate the culture of healthy fish and shrimp larvae.6.There are four main proposed working programs of this plan for the coming year.1.The artificial spawning and rearing of the larvae of Tectus pyramis :To compareamong the methods of stimulating by taking breeders off water, temperature stimulatingand the combination of these two to decide which is more useful to induce spawning. Inthe rearing of the larvae, we use different bases to attach the microalgae to induce thesnail larvae to settle. The larvae then can graze on algae which flourished on the testingbases. The testing bases will be stones, pvc plates and Ulva Lactaca for this year. 2.Thelabeling experiment of hatchery-reared javeniles of T. pyramis :Five labeling methodswill be tested, and these are normal paint, anti-fouling paint, polyeaster, fishing linestuck with polyeaster and shell top abrasion. The efficacies of them will be comparedeach other to find out the best one. 3.The juveniles mid-nursing and release of T.pyramis: The juveniles of top snail will be removed from the nursing room and placedin the beach of Peng-Hu Islands to stock and released to the conservation sea areaaround Peng-Hu Islands.