1. The purpose was to study the bacterial flora and to cleare the bacterial compositionin the digestive tract of shrimps. Here, to investigated the bacterial composition betweennormal shrimp and diseased shrimp in the digestive tract. Our research is to useprobiotic bacteria to add the shrimp culture ponds and shrimp's food to inhibit theVibriosis, and finally to attend the goal of increasing the survival rate of pond shrimps.2. The third year survey on the water quality of mariculture in Pei-men bay will becontinued as passed two years ,bacteria phases summed up to this study in addition ;Thepurpose was , not only to study the basic water quality of aquaculture in Pei-men bay ,but also to increase profit of fish farmer.3. For improving water quality of culture pond, collect precipitation. in the meantime,analyzing and investigating precipitation chemistry ,planton and nutriment of waterquality of fish pond are improvtant for reaching high survival and productivity.4. This paper will describes the activity of phagocytosis in macrophage after tilapiainduced by chitosan intake. Total amount of macrophages showed highly phagocytosis ability will estimate when treated with bacteria in vitro in experimental group. Thenumber of bacteria was phagocytosed by macrophage will also estimate.