The grouper is an economically important food fish in tropical and subtropical areas.Infectious diseases are the major causes of economic loss in grouper culture. Currentmethods for treating or controlling diseases include antibiotics and chemotherapeuticsthat are marginally effective. Vaccines are being using with some fish species; howevervaccines are not effective against many commercially important diseases. Thus, it wasimportant to exploring other methods of preventing disease, including use of variousprobiotics strains to the feed for the express of altering of flora in the gut. This presentstudy was designed to isolate the gut flora and to determine its effects on the grouperwith a goal to enhanced health of grouper.