Rearing experiments will be conducted to investigate the effects of temperature on thegrowth and ammonia-N excretion rate (mg total ammonia-N/kg body weight/h) ofKuhlia rupestris, at three temperature treatments with two replicates per treatment. Theresult of this trial will be helpful for culturing and breeding this local freshwater fish.Theresearch goal would be build the critical apparatus on the reproduction effect for (Spinibarbus hollandi) with the spawning ground、activate area and rate of water flow.