Cobia is a cultured fish with high potential international market. The cobia cultureindustry will be developed as large as salmon culture industry in near futhure. However,disease and use of large quantity of fish oil become the hurdles of their development.Many efforts at molecular level have been put on the salmon culture studies. On theother hand, no such efforts have been focus on cobia. Therefore, cloning and analysis ofcobia actin promoter that are improtant for molecular studies become the objectives ofthis research project.