Recently, the industry of fish hatchery was developed in Taiwan, but many problemsremained to be resolved. Among of them, bacterial diseases and virus are considered tobe a major cause of mortality in fish hatcheries. Therefore, the use of probiotics tocontrol potential pathogens is developed. In this study, probiotics will be used in thelarval culture of grouper, either delivered directly into the water of tanks, or via live foodcarriers such as rotifer, copepod nauplii to inhibit the vibrio spp.