A high performance liquid chromatographic tandem mass spectrometric (HPLCMS/MS) method was used in this study with the aim of developing a multiresiduemethod allowing for the same chromatographic system for the separation anddetermination of drugs banned and used in the aquatic animals. The procedure wastherefore fully optimized for the separation of drug standard mixtures using a gradientelution on a reversed phase HPLC column. The optimization for the detection of drugsby HPLC-MS/MS was investigated. Multiple reaction monitoring (MRM) wasemployed for quantitative determination. The limits of quantitation and overallrecoveries of the entire method were estimated for each drug in various seafood.