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一.餌料生物保種及應用技術研究-附著矽藻在九孔種苗生產之應用本研究從九孔苗適用附著矽藻之分離、培養技術之建立等，發展出利用培養之矽藻來餵養九孔苗，本年度計畫擬探討這些餌藻對九孔種苗培育之影響，建立培養之附著矽藻在九孔種苗培育之應用。二.九孔之SPF餌藻開發研究為減少致病性弧菌(溶藻弧菌)藉由龍鬚菜的投餵，而入侵九孔養殖環境，本研究乃試驗數種消毒方法(滅菌海水清洗、滅菌淡水清洗、臭氧、漂白水)及益生菌對龍鬚菜所帶有之溶藻弧菌的消毒或抑制效果，以建立適合九孔業者在龍鬚菜投餵前的消毒模式。執行內容包括龍鬚菜消毒後之短期蓄養觀察、數種消毒方法對溶藻弧菌的消毒效果評估，及九孔投餵已消毒的龍鬚菜後,九孔體內及九孔池水的菌量檢測。三.優質九孔種貝之培育1.了解不同季節傳統式與室內循環水養殖九孔種貝其腸道中菌量與菌相之差異。2.確立增強種貝的抗病力與免疫力之方式。3.九孔種貝餌料投餵模式確立。4.九孔人工繁殖一年執行10次以上，提供研究單位所需受精卵及種苗。5.生產優質九孔種苗60000粒。四.優質九孔種貝量產之研發與養殖技術之改進九孔養殖由於成功的育種及育苗技術，以然成為我國重要的產業。然而，近年來卻由於弧菌的感染，使得很多育苗場損失慘重，其所表現的主要病症通常是鮑苗無法附著而脫板。九孔就像其他的軟體動物一樣不具免疫球蛋白，主要的防衛系統包括血細胞與體液免疫(溶菌.，凝集素等)兩部份，這些系統在面對病原體的挑戰時都會有所反應。本年度的研究旨在尋找適當的免疫增強物質得以提高的九孔抗病力，我們也會將調查免疫增強物質添加在餌料後餵食種貝後各項免疫反應的變化，希望此有助於由母系效應培養出較健康的幼貝。

一.餌料生物保種及應用技術研究-附著矽藻在九孔種苗生產之應用This study has established the isolation and culture collection of suitable diatoms and theculture technique for those diatoms. This year's project aims for the effects on thediatom diets on the growth and survival of post-larval abalone Haliotis diversicolor inorder to establish application the culture diatoms for larval rearing of this abalone.二.九孔之SPF餌藻開發研究The abalone production in Taiwan has dropped down in recent years because of theserious bacterial infection, especially the Vibrio alginolyticus. The V. alginolyticus canbe easily brought to the abalone culture ponds by feeding seaweeds (Gracilariatenuistipitata). For preventing pathogenic Vibrio infection, this study aims to test thedisinfection effects on G. tenuistipitata by disinfectants or probiotic. Disinfectantsincluding sterilized seawater, fresh water, sodium hypochlorite or ozone are used to washthe seaweed. The growth of G. tenuistipitata and total bacteria and Vibrio counts aftervarious treatments are used to evaluate the suitable procedure for disinfection. Inaddition, the total bacteria and Vibrio counts within the abalone guts and in its culturewater after feeding the treated G. tenuistipitata are used to evaluate.三.優質九孔種貝之培育1.To understand the differences in the abundance and diversity of intestinal bacteria indifferent seasons betweentraditional and interior circulating system.2.Establish the method to increase the resistance to disease and immunity.3.Establish the feeding model for brood abalone.4. To execute artificial reproductions of abalone above 10 times a year and tosupplyzygote and larvae for research purpose requirement.5.A total of 60000 abalones were produced.四.優質九孔種貝量產之研發與養殖技術之改進Culture of small abalones, Haliotis diversicolor has expanded quickly due todevelopment of successfully propagation and larviculture. However, in recent years,farmers have experienced mass mortality of abalone reared in the grow-out ponds. Theabalone larvae often can't success to settle in the nursery stage. The bacteria, Vibriospp., have been documented to caused vibriosis leading to death of abalone in warmerwater temperature.Abalone does not possess immunoglobulins. The major roles of defense systems inabalones are hemocytes and humoral systems (lysosomal enzymes, lectin, etc.). When theabalone is challenged with the virus and bacteria, the immune systems will response toface the infection. Herein, we try to find some immunostimulants, which can increasethe defense activity of abalone. We also investigate the effects of immunostimulants onthe immuno responses of stock abalone. Through the maternal effect, we could obtainbetter healthy larval abalone.