Groupers are protogynous hermaphroditic fish species. Due to the uneasy acquisition ofmature male fishes because of their large size and old age, the lack of male fish hasalways been the important problem of artificial propagation of groupers. However, sincethe establishment of the artificial transsexual and breeding techniques, the production ofgrouper seedlings have grown to a commercialized mass production scale, but it has theproblem of low stability. The major reason is that virus factor as nervous necrosis virus(NNV) and TGIV in recent years (Chi et al., 1997; Jhao et al., 1997), which lead to alarval breeding rate of less than 1%.To solve the current problem of virus infection among groupers, production of virus-freeeggs is the short-term solution; disinfection of the ponds, water, tools and feedstuff andquarantine of viruses, together with detection of viruses, are the measures that theindustry may adopt immediately to reduce the chance of virus attack. In the long run,development of SPR seedlings, together with the development of vaccine, and theproduction of broodstock with strong anti-virus ability are the fundamental solutions.Therefore, this project starts from the breeding of seedlings; healthy and virus-freebroodstock are selected and their immunity is boosted with strengthened nutrients forthe production of high-quality fertilized eggs, in order to solve the problem of low andunstable survival rate of grouper larvae now prevalent in the industry.