In experiment 1, the reproductive performances of pond (100 m2 concrete pond) cultured and tank (20 m2 FRP tank) cultured female broodstock of Penaeus monodon were compared over two months period after eyestalk ablation. The results were as following: number of spawning per female was 3.0 and 3.6, total eggs per female was 1.09 and 1.19 million, eggs per spawning was 362 and 331 thousand, highest number of spawning among females was 9 and 10, highest total number of eggs produced by a single female was 4.03 and 4.35 million, highest number of eggs produced by a single spawning was 832 and 777 thousand, respectively. The above results indicate that pond cultured female broodstock of Penaeus monodon had the same reproductive performances as pond cultured ones. In experiment 2, the reproductive performances of tank cultured female broodstock of Penaeus monodon co-cultured with grouper or not were compared over two months period after eyestalk ablation. The results were as following: number of spawning per female was 4.3 and 2.8, total eggs per female was 1.34 and 1.02 million, eggs per spawning was 341 and 369 thousand, highest number of spawning among females was 10 and 5, highest total number of eggs produced by a single female was 4.34 and 2.72 million, highest number of eggs produced by a single spawning was 669 and 777 thousand, respectively. In experiment 3, the reproductive performances of tank cultured female broodstock of Penaeus monodon co-cultured with Perinereis mictodonta or not were compared over one month period after eyestalk ablation. The results were as following: number of spawning per female was 1.8 and 0, total eggs per female was 454 and 0 thousand eggs per spawning was 247 and 0 thousand, highest number of spawning among females was 4 and 0, highest total number of eggs produced by a single female was 1.63 and 0 million, highest number of eggs produced by a single spawning was 330 and 0 thousand, respectively. The above results indicate that pond cultured female broodstock of Penaeus monodon co-cultured with either grouper or Perinereis mictodonta had higher reproductive performances.