Cage-cultured cobia juveniles are susceptible to Photobacterium damselae subsp. piscicida, resulting in low survival rates. The purpose of this study is to investigate the disease resistance to Photobacterium damselae subsp. piscicida of F1 progeny, obtained from broodstock cultivated from wild-caught cobia juveniles. Results show that the disease resistance of F1 progeny in the wild broodstock line is stronger than those of the inbred line. Mortality in the F1 progeny still occurred when infected with high concentrations of bacteria, although this occurs later than those from the inbred line after infection. This study shows that producing disease-resistant progeny from wild broodstock is an important strategy in improving the low survival rate of cobia juveniles in the cage-culture stage.