Feminization of the Nile tilapia (Oreochromis niloticus) fry, reproduced by male YY crossed with female-reversed XY, was implemented. The fry were fed with eel feed mixed with 100 ppm 17α\-ethynylestradiol for 59 days, each day two times, then fed with perch feed only. The grow-out 234 tilapias were with 184 females and 50 males, sex-reversed rate was 78.63%.Keywords: genetically male tilapia, feminization