The matured king grouper brookstock induce spawning by HCG and LHRHa from China and HCG (Gonad) from Taiwan, respectively. The same dosage was injected in this experiment. The spawning rate was 0% in China system, but 100% in Taiwan system.The larvae of king grouper and coal trout infected NNV was checked by RT-PCR. They was used experimental animal to carry out nursery trial. The result of survival was 26.2% in king grouper and 67% in coal trout after 30 days trial..