For the tank culture of macroalgae as sea-vegetable for human, the first step for mass culture is the clean and health germlings. Therefore, the target algae were collected from the wild and aquaculture farms. The techniques for long term culture maintenance were established for 7 Caulerpa species, three Gracilaria species and one Ulva species. The preliminary experiments for the growth condition of Caulerpa microphysa, Ulva lactuca and Gracilaria tenuistipitata var. liui germlings to evaluate their potential for mass culture were conducted. The results showed the C. microphysa maintained in suitable condition cultured at the optimal environments (28-30 psu、100-200 μE、25-28 ℃) could grow better, with editable parts longer than 15cm and constant and higher yield than in the wild. The juveniles U. lactuca grew from the spores cultured in the outdoor tanks at density of 1 kg/m2 achieved double weight gain in 7 days. The lateral branches of G. tenuistipitata var. liui could be used as seed for mass cultivation, specific growth rate range from 8.8 to 10.3 %/d, more than 100 tiny newly-growth lateral branches ( 3-5 mm）grew up to 3 kg full size Gracilaria with no epiphytical contamination occurred.