The size of protozoa is often smaller than rotifer and copepod nauplii could be suitable as food sources for the smaller mouth of marine fish larvae. Therefore, the aim of this study is to develop a mass cultural technique of protozoa Ciliate, Euplotes sp. was isolated from the culture pond outdoors and pure culture in the laboratory. To develop a mass cultural technique of protozoa, artificial food was only used as the food for this experiment. The culture was carried out indoor on 200 L plastic tank at water temperature 27~ 30℃ and salinity of 30 psu. The initial density of Euplotes sp. was 250 cells/ml. The continuous cultural period was over 47 days and average production between 2000 and 20000 cells/ml. The result showed that it could obtain stable production of Euplotes sp.In the future study, the aim is to utilize probiotics, microalgae and organic matter via living food carriers such as protozoa in the larval culture of marine fish to promote the survival rate of larvae.