跳到主要內容區塊

黃蠟鰺魚塭種魚培育之研究

  • 日期:98-04-20
  • 年度:2008
  • 領域:漁業領域
  • 主持人:朱永桐
  • 研究人員:葉信利、朱永桐、林峰右、張丁仁、梁貴龍

黃蠟鰺以型態分類學辨別,主要分為兩種(1)短鰭黃蠟鰺(TrachinotusOvatus)又稱卵形鯧鰺(2)長鰭黃蠟鰺(Trachinotus blochii)又稱布氏鯧鰺;經實驗數據得知,長鰭或短鰭黃蠟鰺可經由形態上之差異明顯辨別區分,並利用Cytochrome b gene為鑑別指標則可以更清楚其鯧鰺屬之間親緣關係,另外生殖腺組織切片可觀察生殖細胞發育情形。因此本計劃目的主要針對陸上魚塭培育與箱網養殖黃蠟鰺種魚在性腺成熟度差異現象做探討,主要藉由(一)形態學及基因鑑種相關技術,建立長、短鰭黃蠟鰺魚種辨識(二)生殖腺組織切片,比較陸上魚塭培育與箱網養殖性腺成熟度之差異(三)環境及水文資料為評估指標;實驗結果顯示長鰭及短鰭黃蠟鰺在形態上,脊椎骨數都為23,在背鰭前髓棘間骨部分,長鰭黃蠟鰺為卵圓形而短鰭黃蠟鰺為倒L型,另外在第二背鰭最長鰭條與臀鰭最長鰭條,長鰭黃蠟鰺比短鰭黃蠟鰺長,是為明顯特徵,顯示在形態上可明顯看出差異。在基因選殖部份利用 Cytochrome b gene高度保留區所設計引子,成功增幅長鰭與短鰭黃蠟鰺376鹼基對(bp),最後觀察在9-12月秋季,生殖腺組織切片比較4年齡陸上魚塭培育與箱網養殖黃蠟鰺結果,其細胞發育為週邊核仁期(perinucleolus stage)。

研究報告摘要(英)


The Pompano (Trachinotus ovatus) in the traditional classification divides into Trachinotus ovatus (short fin) and Trachinotus blochii (long fin). In 1989, Taiwan started to gather raises the Pompano, the fry mainly fishing for north sea area. pond culture for many years, discovered that the Trachinotus ovatus growth was quick and cold resistant. This study has three major terms: First, established the platform to recognize the Trachinotus ovatus (short fin) and Trachinotus blochii (long fin) by Taxonomy and gene identifying skills. Second, reproduction glandular histological, compared the germ cells maturity difference with Cultivation and Net Cage. Third, investigation environment and the hydrological data take the appraisal target. The experimental result showed that both Trachinotus ovatus and Trachinotus blochii spine number are 23, and the dorsal hard fin number Trachinotus blochii is 5, Trachinotus ovatus is 6. Moreover, a apparent characteristic is the Trachinotus blochii first soft dorsal fin is longer than Trachinotus ovatus, the expression may by the shape characteristic obvious resolution difference。 To design Cytochrome b gene degeneration primer for Trachinotus ovatus (short fin) and Trachinotus blochii (long fin). Successful to sequence for 370 bp region of the mitochondrial cytochrome oxidase subunit I gene. 4 age Pompano breed in Cultivation and Net Cage, reproduction glandular tissue discovered that the cell growth in perinucleolus stage, the germ cells has not reached maturely.