In the global tendency of gradually reducing the use of antibiotic, this research discusses the extract of functional material from seaweed products and herbs. It is to carry on the screening antibacterial material from herbs or using algae as raw material, and to try the lactobacillus fermentation algae to use as prebiotics, as well as to assess the applied method and effect of candidate probiotics, Bacillus cereus biovar toyoi and B. fusiformis which were isolated from hydrogen-producing fermented solution, using in the larviculture system of Penaeus monodon and Litopenaeus vannamei reared from zoea 1 to postlarva 1, to develop immune ability functional and the antibacterial feed supplement, to provide antibiotics or the medicine substitution for aquaculture farmers, that may prevent fish disease and enhances the security of aquatic products.