The objective of the project is to establish the technology of zero-exchange cutltre of SPF white shrimp, Litopenaeus vannamei in small tank. Parameters related to the technology will be collected in order to establish the "organic reused zero-exchange culture technology". The results indicate that, under zero exchange culture condition, the best culture density is 200/m2 and the best salinity is 25 ppt。Application of plastic mats decrease ammonia nitrogen by 64%. Comparing to exchange, zero-exchange not only save water usage by 80%, but also lower FCR by 5%. Application of net pockets not only remove suspended solids by 75% but also increase yield by 54%, lower FCR by 22% further. Overall, using this technology, the water usage and discharge, feed and culture cost will reduced by 90%, 26% and 13%, respectively.