The hatchery of oyster was always built depend on the demands of farmers themselves in Taiwan before. Thus, the standard operation protocols of the hatchery of oyster are absent here now. Because of the potential industry of cultchless oyster in Taiwan and being capable of culturing in the ponds on the lands, it is necessary to setup the Oyster Seed Hatchery. We integrate the techniques developed recently in our laboratory to establish a hatchery that is useful to practice the artificial propagation, spat settlement and related research. The average setting of eyed larvae of five substrates, black plastic bag, feed bag, transparent sheet, PVC sheet and black net, were 33±8, 22±11, 8±3, 6±4 及0±0 individuals per sheet, respectively.. The darker the substrate is, the better the setting rate is, except the black net is due to the large aperture. In the study of the optimal rate of eyed larvae to oyster shell, it was found that the setting rate of 200, 100, 50 eyed larvae to one shell were 7.6, 2.1, 1.0 spats per shell, respectively. The more the eyes larvae are put in, the better the setting rate. But it is necessary to do further study to get more setting rate.