Limulus amoebocyte lysate (LAL) clots when mixed with of endotoxins which are lipopolysaccharide components of the outer cell-wall layer of gram-negative bacteria. Since the initial description of the lysate method for the detection of endotoxins, it has become an international standard method. Although Jinmen and the Penghu coast has the wild Chinese horseshoe crab, but not yet developed into the commercialization of biological agents. With the depletion of marine resources, wild king crab production declining, the future cultured crab would become the tendency. However, the culture of Chinese horseshoe crab needs 10 ~ 15 years to adult, it would increase the difficulty of culture. In addition, the blood components have obvious seasonal variation, with seasonal changes in the composition of the blood there will be significant difference. So it would not to collect Limulus blood throughout the year and affect its biological agents and yield sensitivity.The results on the amoebocyte cells from Tachypleus tridentatu showed that seasonal variation and a difference of up to 1.5 times. Estimation total hemolymph volume in the horseshoe crab Tachypleus tridentatus and endotoxin detection kit freeze-drying preservation.