The purpose of this investigation is to successfully rear yellow fin tuna (Thunnus albacares) breeder candidates and develop tuna industry-oriented culture techniques. At present, 53 tuna (2-4 years old) are culture in a 1500 m3 round concrete recirculating tank system. The daily feed weight has gradually increased from 5 kg per day in January to 15.5 kg per day in October. Throughout all this study, ranges of water quality parameters were: temperature, 27-30°C; total alkalinity, 261-294 mg L-1; total ammonia nitrogen (TAN), 0.01-0.15 mg L-1; Nitrite (NO2-N), 0.01-0.04 mg L-1; Nitrate (NO3-N), 9.09-19.69 mg L-1 and phosphate, 0.196-0.997 mg L-1. For handling and blood sampling procedure, tuna fish were anesthetized but still resulting in high mortality rate (>50%). Additionally, 39 (1 year old) tuna were stock with older tuna in same tank. Due to size differences, survival rate of 1-year old tuna was 56%. Recirculating systems for stocking yellow fin breeders has shown promising results. We expect to have natural spawning of yellow fin tuna in recirculating pond systems.