The purpose of this studies will defined the activity of macrophage phagocytosis after eel (Anguilla japonica) induced by immunostimulant agents (chitosan and β-glucan). The result of challanged test showed that 2%β-glucan group fish possessed the best protectively ability, and showed strongly bacterial phagocytosed ability in vitro condition. Moreover, β-glucan were used as bacterial vaccine adjuvant is useful.