In 2006,The germ cell from cultivated broodstock, carry on the viral PCR examination in view of Taiwan Grouper Iridous virus (TGIV). The result showed the broodstock did not to receive the TGIV infection. This year in July and in October aims at nerve necrosis virus (NNV) to carry on the viral PCR examination; The result showed breeding fish not to examine the nerve necrosis virus , too. The male giant grouper mature in June to November; the female mature in July to November, both mature peak in July to August, that investigation from Tainan station in 2006. Sampling of male had half number of maturely in this year July, but the female only has 20% maturely. Mid-October, female has 53.33% maturely and male were all maturity. We selected 6 female and 6 male to induce spawning and obtain the embryo egg 539 g.