The aim of the research investigated the effects of the probiotic, a photo-synthetic bacteria Rhodovulum sulfidophilum, on the coliform and pathogens in shrimp aquaculture. The results indicated that Vibrio alginolyticus and V. parahaemolyticus were inhibited by the probiotic when it was added in the ponds of shrimp larvae. But this inhibition can’t be found in the plate of E. coli. The amounts of ammonia-N and nitrite-N in the ponds also mitigated by adding photo-synthetic bacteria. In addition, the bacteria stabilized pH values in the water. Above all, treatment with photo-synthetic bacteria increase survival rate of the shrimp.