The fifth generation of local SPF tiger shrimp Penaeus monodon without WSSV and TSV infection were picked up via PCR examination and observation, and 151 female and 100 male were given artificial induced maturation, and reared 330 thousand of the sixth local tiger shrimp larvae. Selfcross between the hybrids from Taiwan F3♀ and Malaysia P♂ (F1) was carried out, and then sieved out 68 female and 40 male from them. In addition, 101 Taiwan SPF F5 female tiger shrimps were hybridized again with 40 male of the cross hybrids F1. There are 180 thousand F2 shrimp larvae of the selfcross between F1 hybrids, and 110 thousand F2 shrimp larvae of the hybridization between Taiwan F5 and hybrids F1