農業部-水產試驗所全球資訊網農業部-水產試驗所全球資訊網

一般原生動物的體型比輪蟲、橈足類幼生更小，應適合口徑小的海水魚苗之初期餌料生物，本研究乃探討其培養技術與應用於魚苗培育效果。取自室外養殖池，經分離純化培養的原生動物纖毛蟲的游仆蟲(Euplotes sp.)，探討使用不同的餌料培養效果。 以三種不同的餌料，人工飼料醱酵液、等鞭金藻(Isochrysis galbana)和麵包酵母，每組三重複。在溫度25 ± 1℃，塩度25psu下，餵飼游仆蟲，最初密度為10隻/ml，48小時結果顯示，人工飼料醱酵液培養的數量為3090 ± 856隻/ml，其次以麵包酵母為1110 ± 221隻/ml及等鞭金藻為163 ± 31隻/ml，對照組為11 ± 4隻/ml，顯示人工飼料醱酵液較適合為游仆蟲的餌料。以人工飼料醱酵液培育出的游仆蟲進行點帶石斑 (Epinephelus coioides)仔魚培育，於孵化3日後，再添加餌料游仆蟲，密度為3隻/ml，每組三重覆，本實驗期間共計7天，初步結果顯示游仆蟲適合點帶石斑的初期餌料。

Recently, the industry of fish hatchery was developed in Taiwan, but many problems remained to be resolved. Among of them, bacterial diseases,virus or the size of living food are considered to be a major cause of mortality in fish hatcheries. Therefore, the use of probiotics to control potential pathogens is developed. In this study, the aim is to utilize probiotics ,microalgae and organic matter via living food carriers such as protozoa, rotifer or copepod nauplii in the larval culture of marine fish to inhibit the vibrio spp.and promote the survival rate of larvae.The size of protozoa are often smaller than rotifer and copepod nauplii as alternative food sources for the smaller mouth of marine fish larvae. The aim of this study is to develop a cultural technique of protozoa and used for the fish larvae rearing experiment. Ciliate, Euplotes sp. was isolated from the culture pond outdoors and pure culture in the laboratory. To develop a cultural technique of protozoa, three kinds of food as following: artificial fermented liquid, bread yeast and microalgae (Isochrysis galbana) were used as the food for this experiment. Each treatment was three triplicates. The cultures were carried out on 500 ml glass beakers at the water temperature 25 ± 1℃and salinity of 25 psu. The initial density of Euplotes sp. was 10 cells/ml The result showed that the total average production of treatments and control is 3090 ± 856, 1110 ± 221 , 163 ± 31 and 11 ± 4 cells/ml, respectively. In conclusion, Euplotes sp. grows fast and attains high densities in feeding artificial fermented liquid treatment.The contribution of ciliates as the first living food source to survival of first feeding red-spotted grouper larvae, Epinephelus coioides, was examined in rearing experiment. The larvae were exposed to a ciliate, Euplotes sp. at the density of 3 cells/ml plus control without ciliates. Higher survival of the larvae over the first 6-7 days was observed by feeding Euplotes sp. The result suggested that ciliates Euplotes sp. could be as alternative food sources to larger living food by promoting the survival of fish larvae.