The present study had investigated the relationship between tuna longline fishery and spatio-temporal variations of Kuroshio Current derived from multi-sensor satellite data. The preliminary results were listed as follows: (1) The 1,225 NOAA/AVHRR sea surface temperature (SST) data, 1,331 EOS/MODIS SST data, 696 EOS/MODIS ocean color (chlorophyll-a concentration) data, and the 120 monthly average TOPEX/Poseidon sea surface height anomaly data had been applied and transferred into the ArcGIS point or raster layers in this study. (2) Analyze to the Kuroshio Current velocity measured by the Argos satellite buoys from 1987 to 2006 had shown that the larger average current velocity was 125-150 cm/sec, found in Tai-Dong coastal water during the summer time. Its maximum value was 170 cm/sec. Conversely, the less average current velocity was 75-100 cm/sec, found in Su-Ao coastal water during the winter time, its maximum value was 101 cm/sec. (3) The Sb-ADCP data from 1991 to 2007 was examined to find out the maximum current velocity happened in the summer time from surface to 200m water depth in the Kurosio Current waters off eastern Taiwan. The covering width of Kuroshio Current defined by the high current velocity more than 50cm/sec was about 75 nautical miles. The seasonal Sb-ADCP current vectors also showed the Kuroshio surface and subsurface water usually intruded onto the continental shelf of East Chin Sea in the winter time. (4) The seasonal variations of Kuroshio main stream was examined by the Argos and Sb-ADCP data, and showed that its main axis closed to the eastern Taiwan in the winter time and left for offshore in the summer time. (5) In this study, the Kuroshio Current Chlorophyll Front (KCCF) was defined to be a useful ecological indicator. And the longitudinal migration of the KCCF was evident, found in the eastern Taiwan coastal waters. However, the remarkable latitudinal migration of the KCCF was found in the southern Japan coastal waters.