In this research, yolk of immunoglobulin against TGIV was prepared and its effectiveness on passive protection. Massive multiplication grouper iridovirus of Taiwan (TGIV ) by the grouper swimming bladder cell cultured.In order to get adequate TGIV antigen ,the concentration used PEG (Polyethylene glycol) and purification from the sucrose discontinuous gradient ultracentrifugation. The chicken immunized by intramuscular injection with purified TGIV, and to analyze the IgY antibody titer by ELISA method, the result in laying egg start at three months after immunization .The titer of IgY in yolk were between 102 to 103.